SKU: 44440804414

ZymoScript One-Step RT-qPCR Kit

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Description

ZymoScript One-Step RT-qPCR KitZymoScript One Step RT qPCR Kit contains all the necessary components for fast and robust RNA quantification in a single kit. Room temperature reaction setup is quick and easy. The ZymoScript One Step RT qPCR Kit eliminates the need to transfer tubes between reverse transcription and qPCR, which minimizes sample handling and reduces the risk of contamination. The included Reaction Buffer (4X) contains dNTPs, MgCl2, and other additives to ensure

ZymoScript One-Step RT-qPCR Kit contains all the necessary components for fast and robust RNA quantification in a single kit. Room-temperature reaction setup is quick and easy. The ZymoScript One-Step RT-qPCR Kit eliminates the need to transfer tubes between reverse transcription and qPCR, which minimizes sample handling and reduces the risk of contamination.

The included Reaction Buffer (4X) contains dNTPs, MgCl2, and other additives to ensure complete cDNA synthesis and specific qPCR amplification in just 1.5 hours. Non-interfering blue tracking dye is included to simplify reaction setup and prevent pipetting errors. The 20X Enzyme Mix containing temperature-activated reverse transcriptase, hot-start DNA polymerase, and RNase inhibitors ensures maximal specificity. The ZymoScript One-Step RT-qPCR kit is compatible with both SYBR-Green and TaqMan probe-based assays for ultimate flexibility. A 20X Fluor Dye for SYBR-Green based detection methods is included for added convenience. Detection of multiple targets in a single reaction (multiplex RT-qPCR) is feasible using TaqMan probes (not provided).

The ZymoScript One-Step RT-qPCR Kit has been rigorously tested to demonstrate superior sensitivity, specificity, and reproducibility. The kit can reliably quantify as little as 0.1 pg RNA (including difficult GC-rich targets) and outperforms other commercial kits. This kit was also used to develop a molecular diagnostic test for COVID-19 ranked third in sensitivity by the American FDA. The unique buffer system and temperature-activated enzymes facilitate the melting of secondary structures and ensure ultimate specificity for the intended product. ZymoScript One-Step RT-qPCR Kit is the product of choice for simple and sensitive RNA quantification. It is the ideal product to use as a building block for RT-qPCR multiplex assays or high-throughput diagnostic applications. For quick and efficient cDNA synthesis and 2-step RT-qPCR options, see ZymoScript RT PreMix Kit (R3012) and ZymoTaq qPCR Premix (E2054).

Zymo Research is ready to support your RNA studies and help simplify your RNA quantification workflow. Please contact [email protected] for more information.

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SKU: 44440804414

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4.3 ★★★★★
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Jewell Urbano
Boise, US
★★★★★ 5
Wow.
Format: Kindle
Okay I’m usually not one for stand-alone’s I’m an avid series reader but my goodness am I so happy I read this! This story was brilliant & so absolutely mesmerizing. I loved reading about each character and their struggles as well as what helped them to move forward. The ending definitely brought tears to my eyes so hard. I truly wasn’t expecting some of what happened in this story. There is about to be a spoiler I am going to reveal so please stop reading if you don’t want the spoiler !!!! ⚠️ ⛔️ ‼️ I loved that the author didn’t do what most authors do with irredeemable male characters. I truly was hoping that Nate Jr. would be apart of the pack after the way he treated Astrea bc he truly didn’t deserve it. Though I must say you did a wonderful job or redeeming him as a person. I cried my eyes out when he walked into the story. I was truly terrified he was going to be a bad guy to the end. However you truly did him such a justice by having him realize his faults & having him redeem himself in the most wonderful way. I’m so sad that he didn’t get to hear how much his brother loved him & forgave him before dying. But again you wrote that ending so beautifully & I just can’t express how much I loved this story & how you took a different route than most authors I have read have. You are a remarkable author Cinder Blaze & I thank you generously for creating such a masterpiece.
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Reviewed in the United States on April 28, 2025
K
Verified Purchase
Kristen Linscott
Whiting, US
★★★★★ 4
Omegaverse
Format: Kindle
I was pleasantly surprised by this omega verse book. This 1 definitely had a few new twists And I really enjoyed reading it. The main female character was a badass and awesome. She had 1 best friend I wish we could have seen a little bit more of their friendship in this book. Her relationship with the male characters was good not to contrived or super instantaneous. And we had some fun plot twists that I didn't expect. I wish we had more of a follow up on the situation with her family her background and her mother who was a wench. I would definitely recommend this book.
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Reviewed in the United States on April 9, 2025
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Verified Purchase
Amanda
Natrona Heights, US
★★★★★ 5
Wow. Just wow. An amazing read
Format: Kindle
This book was eloquently written, or should I say the authors writing style is very eloquent? I loved the characters, and the story was quite compelling. I absolutely loved the FMC, she's a BA who doesn't take any crap & gives as good as she gets. A certain person certainly got his just desserts & then some, the earlier scenes of which were quite satisfying, had me punching the air & everything haha. All in all 20/10, great read
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Reviewed in the United States on November 29, 2024
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Lauren
Battle Creek, US
★★★★★ 3
My opinion
Format: Kindle
Let me preface this by saying I really liked the story and the characters however, this book is in desperate need of some sort of editing. It's not misspelled words or formatting, but continuous run on sentences. Redundancies within the sentences. There were a couple of paragraphs that I had to go back and reread 3 or 4 times. Overall, I'd say it was worth the read.
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Reviewed in the United States on May 29, 2025
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Amazon Customer
Phoenix, US
★★★★★ 5
Beautiful
Format: Kindle
I love omegaverses I had looked at this one multiple times thinking it's just another book about cocky rock star guys that let fame get to their heads and there are parts like that so I wasn't 100% off. I started reading the second book and met the fmc Astraea from the first one,after she had a run in with the aphla from the gym.😁 it gave me the push to stop and read book one first and im so glad I did this book was amazing their was so many characters I fell in love with hoping they will find their happily ever after. the guys were great, the plot was 🎯, and the ending had me 😭😭😭. I was wondering how nate could ever redeem himself, and he did. the last scene with him was sad, but I also felt it was beautiful. thank you to the author for making a beautiful omegaverse book that gave me all the feels. now I'm jumping straight into book two.
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Reviewed in the United States on March 25, 2025

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