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Description
Chicken POSTN/OSF2 (Periostin) ELISA KitThe Chicken POSTN OSF2 (Periostin) ELISA Kit measures POSTN OSF2 in Chicken Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with POSTN OSF 2. POSTN OSF 2 in the sample or standard competes with a fixed amount of biotin labeled POSTN OSF 2 for sites on a pre coated antibody specific to POSTN OSF 2. Free
The Chicken POSTN/OSF2 (Periostin) ELISA Kit measures POSTN/OSF2 in Chicken Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with POSTN/OSF-2. POSTN/OSF-2 in the sample or standard competes with a fixed amount of biotin-labeled POSTN/OSF-2 for sites on a pre-coated antibody specific to POSTN/OSF-2. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of POSTN/OSF-2 in the sample. The concentration of the target substance is inversely proportional to the OD450 value.
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Exchange/Return Notes
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