SKU: 5410134660

Porcine ANG (Angiogenin) ELISA Kit

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Description

Porcine ANG (Angiogenin) ELISA KitThe Porcine ANG (Angiogenin) ELISA Kit measures ANG in Porcine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti ANG antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.

The Porcine ANG (Angiogenin) ELISA Kit measures ANG in Porcine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti ANG antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with ANG bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of ANG in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 5410134660

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David
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Important to Know
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I very much like this study guide. It has helped me study for my exams. Some of my test questions have even come straight from this book. HOWEVER, I do caution anyone who buys this book to double check the answers. I have come across MULTIPLE typos and incorrect answers in the Answer Key. I like the content of this book, but for a professionally printed book, the overall completeness is poor.
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This was a great addition to the textbook. The textbook was awful in my opinion but this study guide really explained it in a easy to understand and direct way. I love the matching, fill in the blank and most importantly the case studies. Really helps you figure out what you may encounter pathophysiology wise when caring for patients.
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Cannot recommend this enough. It really cements the material from the book. If you use this, you will understand the content in the book. I find it incredibly helpful in my program.
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Watson's Caring Science is Cutting Edge! You will begin seeing Watson's Caring Science infused in many organizational structures. Ride the wave you won't regret!
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I REALLY like this book. I’m new to online teaching and it has lots of good information and tips
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Reviewed in the United States on January 9, 2020

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