Mouse LRP6 (Low-density lipoprotein receptor-related protein 6) ELISA Kit

Mouse LRP6 (Low-density lipoprotein receptor-related protein 6) ELISA KitThe Mouse LRP6 (Low density lipoprotein receptor related protein 6) ELISA Kit measures Lrp6 in Mouse Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Lrp6 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.

The Mouse LRP6 (Low-density lipoprotein receptor-related protein 6) ELISA Kit measures Lrp6 in Mouse Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Lrp6 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with Lrp6 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of Lrp6 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.