SKU: 59364281096

Human SPHK2 (Sphingosine kinase 2) ELISA Kit

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Description

Human SPHK2 (Sphingosine kinase 2) ELISA KitThe Human SPHK2 (Sphingosine kinase 2) ELISA Kit measures SPHK2 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti SPHK2 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection

The Human SPHK2 (Sphingosine kinase 2) ELISA Kit measures SPHK2 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti SPHK2 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with SPHK2 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of SPHK2 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 59364281096

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4.6 ★★★★★
Based on 9 reviews
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Verified Purchase
TAngell
Lowell, US
★★★★★ 4
Strong
Size: Medium 20", Style: 3 Knot, Pattern Name: Dog
For a five month old puppy with sharp teeth it’s holding up pretty well.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 27, 2026
S
Verified Purchase
Shaienne
Cuba, US
★★★★★ 5
EXCELLENT QUALITY
Size: Medium 20", Style: 3 Knot, Pattern Name: Dog
I have a German Shepherd/Pointer mix who is 10 months old. He has been in the throes of teething and destructive exploration. He's chewed up clothing and shoes. He chewed a hole in our couch and one in our brand new mattress (He was never left home alone until this point but as my mother came to visit and my boyfriend was back at work, I had no choice but to trust him alone for about an hour a day for 3 or 4 days... he clearly didn't earn that trust). He loves ropes because he can gnaw them, whip them around, and play tug of war or fetch with them at any given time. We love ropes because they keep him occupied and relieve his desire to chew things. Unfortunately, he was destroying rope after rope after rope. Some ropes were worrisome because they would quickly start fraying and falling apart. We would have to help remove strings he has swallowed from his behind while he was out doing his business... My boyfriend's mother picked up a Mammoth TireBiter tire and rope toy for him but it was much too small. Still, he loved it and it stood up very well to his chompers despite being way too small/weak for him. Looking for a larger version of the Mammoth TireBiters, I stumbled across this rope toy and gave it a shot. Let me tell you, I don't think I will ever buy rope toys from any other brand! We have had this rope for over a month and it is still going strong. It does show some small signs of wear and tear but you would swear we bought it last week. It is very strong and durable. It has held up through many long gnawing sessions. It has been put through countless hours of fetch and tug of war with no signs of giving out any time soon. I am so in love with this rope and so is my pup!
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Reviewed in the United States on September 13, 2020
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Verified Purchase
Lilly
Lake Worth, US
★★★★★ 5
Good quality
Size: Mini 10", Style: 3 Knot, Pattern Name: Dog
Small but good quality
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Reviewed in the United States on May 28, 2026
A
Verified Purchase
Amazon Customer
Lowell, US
★★★★★ 3
Only for tug-a-war play.
Size: Small 15", Style: 3 Knot, Pattern Name: Dog, Size: Small 15", Style: 3 Knot, Pattern Name: Dog
This should only be advertised as a tug-a-war dog toy. I gave the toy to our 6 month old puppy and in, not even, an hour it was ripped apart. He’s not big enough for the larger ones yet. Not sure if I’ll try the next size larger.
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Reviewed in the United States on May 12, 2026
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Verified Purchase
Beka
Bozeman, US
★★★★★ 5
Definitely worth the price
Size: Mini 10", Style: 3 Knot, Pattern Name: Dog
Not for heavy chewing. This rope is light and not as rigid as the others. My dog prefers this one over her other ropes and plays with it much more.
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Reviewed in the United States on March 10, 2026

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