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Ships within 48 hours · Estimated delivery Jul 17 - Jul 22
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Description
Human UBE2I (SUMO-conjugating enzyme UBC9) ELISA KitThe Human UBE2I (SUMO conjugating enzyme UBC9) ELISA Kit measures UBE2I in Human Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti UBE2I antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with
The Human UBE2I (SUMO-conjugating enzyme UBC9) ELISA Kit measures UBE2I in Human Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti UBE2I antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with UBE2I bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of UBE2I in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
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