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Description
Rat CYP21A2 (Steroid 21-hydroxylase) ELISA KitThe Rat (CYP21A2) Steroid 21 hydroxylase ELISA Kit measures Steroid 21 hydroxylase in Rat samples. The plate has been pre coated with Rat CYP21A2 antibody. CYP21A2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat CYP21A2 Antibody is added and binds to CYP21A2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CYP21A2 antibody. After incubation unbound Streptavidin HRP is
The Rat (CYP21A2) Steroid 21-hydroxylase ELISA Kit measures Steroid 21-hydroxylase in Rat samples. The plate has been pre-coated with Rat CYP21A2 antibody. CYP21A2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat CYP21A2 Antibody is added and binds to CYP21A2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CYP21A2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat CYP21A2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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