Human DKK4 (Dickkopf Related Protein 4) ELISA Kit
SKU: 8559755093

Human DKK4 (Dickkopf Related Protein 4) ELISA Kit

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Description

Human DKK4 (Dickkopf Related Protein 4) ELISA KitThe Human DKK4 (Dickkopf Related Protein 4) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Human DKK4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DKK4 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each

The Human DKK4 (Dickkopf Related Protein 4) ELISA immunoassay kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human DKK4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DKK4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DKK4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human DKK4. You can calculate the concentration of Human DKK4 in the samples by comparing the OD of the samples to the standard curve.

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SKU: 8559755093

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4.9 ★★★★★
Based on 339 reviews
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Sharon Graff
Los Angeles, US
★★★★★ 5
I adopted a raptor instead of a puppy
Color: Teal
He's a chewer. Nothing is safe from his relentless jaws. I have bought him other things to chew which he ignored, preferring rolls of duct tape, empty flower pots and cardboard boxes. But this gator. Well.... NOt only did he love it BUT he opened the bag himself, got the toy out of the box it came in and then carried it proudly into the house. He has chewed the living life out of it so I ordered him another. Cardboard boxes in my house are breathing sighs of relief.
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Reviewed in the United States on April 6, 2026
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DK
Phoenix, US
★★★★★ 5
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Color: Teal
Dog enjoying them
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Reviewed in the United States on May 25, 2026
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supermommy
Louisville, US
★★★★★ 5
Best Dog Bone
Flavor Name: Beef, Size: Large
This is one of the best bones I've seen and I have always lived with dogs. The shape makes it so ergonomic for the dog, both of mine hold the bone down with both paws then chew the part standing up. One of my dogs was able to enjoy this while wearing a cone after surgery, supporting her natural behavior and keeping her busy. These are great for our 11 month old puppy because he was struggling to figure out how to brace the bone without it rubbing his paws, so was bracing on furniture and getting in trouble. Now he can hold the T part easily and doesn't need to use other objects he shouldn't chew by accident. They are durable, too. I got these about 2 months ago and they are barely worn with a rotty/pit/Shepard mix male puppy. If you know dogs, you know that means this is a solid durable bone! I will for sure keep these on hand for years as they are a preferred chew in my house!
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Reviewed in the United States on May 1, 2026
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Baibhav Bhattarai
New York, US
★★★★★ 5
Doggy Delight
Flavor Name: Beef, Size: Medium
Ever felt like your dog’s chew toys are on the fritz, ending up shredded like yesterday’s sandwich? The SPOT Bam-bones PLUS T Bone is here to save the day, offering a durable and tasty solution for aggressive chewers and teething puppies alike. These bamboo fiber and nylon chews are like the super-tough treats your dog dreams of, providing hours of gnawing joy without the splintering mess. My bulldog, who treats his chew toys like they’re part of a survival game, has found his perfect match in these T Bones. The beef flavor is irresistible, making them a favorite among picky pups and ensuring they stay engaged without turning into edible confetti. The 6-inch size is perfect for keeping his jaws busy without overwhelming his mouth – a win-win for both of us. The non-splintering design is a blessing, preventing any painful encounters with broken chews and keeping our home safe from unexpected trips to the vet. Plus, the durable construction means these chews last longer than my patience during a doggy tantrum – and that’s saying something! However, the T Bones can sometimes feel like they’re too tough, requiring a bit of extra chewing to fully enjoy – turning every chew session into a mini workout for your dog’s jaws. Additionally, the price point is slightly higher than standard chews, but the durability and quality make it worth every penny. Overall, the SPOT Bam-bones PLUS T Bone is a fantastic choice for dog owners looking to provide their pets with a long-lasting and enjoyable chew experience. It’s tough, tasty, and built to withstand even the most enthusiastic chewers. A solid 5-star rating for its durability and flavor, with a tiny star lost to its occasional toughness challenges.
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Reviewed in the United States on October 23, 2024
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Amazon Customer
Massapequa, US
★★★★★ 5
The Ultimate Solution for Power Chewers!
Flavor Name: Beef, Size: Medium
I have a Pitbull mix who can usually destroy "tough" toys in minutes, but the Bam-Bones Plus is a total game-changer. The bamboo and nylon blend is incredibly dense; it satisfies that natural urge to chew without splintering like real wood or falling apart like rubber. It wears down slowly and safely, giving my dog weeks of use rather than minutes. ​Smart, Functional Design ​The T-bone shape is brilliant because of the "paw-grip" base—it makes it so easy for my dog to hold it steady while she works on it. I’ve also noticed her teeth looking much cleaner since we started using this, as the raised textures act like a natural toothbrush. Plus, the infused beef scent keeps her interested much longer than a plain nylon bone ever could. ​Final Verdict ​This is a must-have for any owner of a large or aggressive chewer. It’s safe, long-lasting, and provides excellent mental stimulation.
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Reviewed in the United States on April 16, 2026

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