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Description
Caspase 2 Activity Detection KitProduct Specification Usage Determination of pNA standard curve: 1. The 10 mM pNA standard was diluted with reaction buffer to 200, 100, 50, 25, 12. 5, 6. 25, 3. 125 M. Set the zero tube without pNA. 2. Take 100l of each concentration and add it to a 96 well plate or 100l cuvette, and measure the absorbance OD value at 405nm. 3. The OD405 value of each concentration standard tube is the x axis, and the corresponding pNA concentration is the y axis.
Product Specification
| Usage | Determination of pNA standard curve: 1. The 10 mM pNA standard was diluted with reaction buffer to 200, 100, 50, 25, 12.5, 6.25, 3.125 μM. Set the zero tube without pNA. 2. Take 100μl of each concentration and add it to a 96-well plate or 100μl cuvette, and measure the absorbance OD value at 405nm. 3. The OD405 value of each concentration standard tube is the x-axis, and the corresponding pNA concentration is the y-axis. Use Excel to make a standard curve of pNA concentration versus OD405 value. Detection of Caspase 2 enzyme activity: 1. Take out an appropriate amount of Ac-VDQQD-pNA (2mM) and place it on an ice bath for later use. 2. According to the 100ul reaction system: blank control group: 40μl of detection buffer, 0μl of sample to be tested, 50μl of lysate, 10μl of Ac-VDQQD-pNA (2mM); Sample group: 40μl of detection buffer, 50μl of sample to be tested, 0μl of lysate, 10μl3 of Ac-VDQQD-pNA (2mM). Add Ac-VDQQD-pNA (2mM) and mix well. Be careful to avoid bubbles during mixing. Incubate at 37 º C for 60-120 minutes. A405 can be measured when the color change is obvious. If the color change is not obvious, the incubation time can be appropriately extended, or even overnight. 4. The A405 of the sample minus the A405 of the blank control is the absorbance produced by pNA catalyzed by caspase 2 in the sample. By comparing the standard curves obtained in the above steps, it is possible to calculate the amount of pNA catalytically produced in the sample. |
| Theory | Based on the caspase 2 specific hydrolysis of peptide substrate Ac-VDQQD-pNA (Val Asp Val Ala Asp p nitroanilide), the released free nitroaniline pNA has a maximum absorbance at 405nm. Thus, the activity of caspase 2 can be detected by absorbance. Suitable for detecting Caspase-2 activity in mammalian tissues and cells. |
| Experiment Reagent Instrument | Microplate reader or spectrophotometer |
| Synonym | ch-1,Nedd-2,caspase-2,caspase 2 |
| Detection Type | Mammalian tissues, cells |
| Description | Caspase 2 activity detection kit is a kit that detects caspase 2 enzyme activity or purified caspase 2 enzyme activity in cell or tissue lysates by spectrophotometry. Caspases are a family of proteases that play an important role in the process of apoptosis. Caspase 2 can be activated during signal transduction of apoptosis. Caspase 2 mRNA can be cleaved into two different length forms. The full-length mRNA product of Caspase 2 can promote apoptosis, while the short mRNA protein product can inhibit apoptosis. In vitro, caspase 2 can be activated by caspase 1, caspase 3, and granzyme B. |
| Storage Temp. | When stored at-20 °C, Ac-VDQQD-pNA and pNA need to be stored in the dark. |
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4.0 ★★★★★
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★★★★★ 5
this is a totally recommended read. Very unique collection of stories by many ...
Format: Hardcover
This book is one of the must-haves collections for Batman. this is a totally recommended read. Very unique collection of stories by many of the top writer in the comic book industry.
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Reviewed in the United States on June 3, 2015
★★★★★ 5
A must have for any CEO, Entrepreneur, and Product / Project Manager
Format: Hardcover
I'm almost through finishing this book, and I'm already recommending this to so many of my friends.
I feel like many companies could probably save fortunes by reading this book instead of hiring consultants from many top tier firms. You could essentially take this book and follow it as a step-by-step tutorial for making any project succeed. For example, Simon discusses in one chapter the 10 steps for a successful strategic project portfolio management process: know what you have, build momentum, define business goals, capture ideas, be transparent, prioritize, use efficient decision making, establish communication frameworks, conduct postmortems, and improve continually. These may sound like no brainers, but you'll be surprised how many people stray from these steps and lose their focus when they're caught in the excitement of developing their ideas.
If you're an entrepreneur with or without an MBA, this book will help you strategize and plan the next steps after you've settled on your block buster idea. If you're a CEO or you're managing projects within a company, you'll learn in addition the early stages of generating the perfect balance of ideas and resources for creating a portfolio of projects with the highest chance of success.
Amazing book. Learned a ton so far. It's written very well and gets straight to the point. Looking forward to other books by Simon Moore. Keep up the great work.
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Reviewed in the United States on December 15, 2009
★★★★★ 4
guding managers at connecting the portfolio with organsation success
Format: Kindle
good practical description of methods and tool how to connect organizatioa success with good startegic approach which includes poject portfolio.
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Reviewed in the United States on January 20, 2013
★★★★★ 5
I had an interview with Simon
Format: Kindle
I didn't get the job, but I did find his book extremely useful. During my interview, I failed to explain a complex financial concept in way that was easy to understand. Simon does not have this problem. The book was easy to follow and motivated me to drive idea generation within the company I currently work for. I would recommend this book to start ups or innovation officers as I have already seen results.
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Reviewed in the United States on June 30, 2014
★★★★★ 5
Excellent reference for industry
Format: Hardcover
Brilliant handbook for anyone involved in project management work at any level, but particularly for those with a strategic angle. Quickly dispelled the notion that I've been employing best practice, and pointed me in the right direction about putting together a more effective project selection process. The author clearly has a great deal of working experience of portfolio management, and that means that rather than being a dry text it's a very practical guide. Highly recommended.
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Reviewed in the United States on November 9, 2009