Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Jul 23 - Jul 28
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human KAT2B ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a K-Acetyltransferase 2B (KAT2B) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of K-Acetyltransferase 2B (KAT2B) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human K-Acetyltransferase 2B ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | K-acetyltransferase 2B (KAT2B), also known as p300/CBP-associated factor (PCAF), is a protein encoded by the PCAF gene. It is a p53-associated gene and transcriptional coactivator. Its several domains can act independently or synergistically to achieve its functions. It possesses separate acetyltransferase and E3 ubiquitin ligase domains, as well as a bromodomain for interaction with other proteins. It also possesses its own acetylation and ubiquitination sites. The protein encoded by this gene associates with p300/CBP. It exhibits in vitro and in vivo binding activity to CBP and p300 and competes with E1A for binding sites within p300/CBP. It exhibits histone acetyltransferase activity and is associated with core histones and the nucleosome core particle, suggesting a direct role for this protein in transcriptional regulation. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.6 ★★★★★
Based on 20 reviews
Sort
Product Reviews
★★★★★ 5
JAXPETY Room Divider Panel 6Ft Privacy Screen Wall Divider 88" W x 73" H - 3 Panel Black
Color: Black, Size: 3-Panel, Color: Black, Size: 3-Panel
I was looking for a screen to use while making video calls. I am on a budget, wanted something easy to put together, and would hide my apt in the background. Being on a budget, I thought my purchase qualified for the coupon. I was mistaken but contacted the seller. I received a quick response explaining how the coupon was applied. I was happy with the quick response and explanation which I verified when I went back to the page.
The once all put together the screens are light weight and fairly easy to move. they do not seem to be made for easy daily breakdown. However, if you should need to take them apart to move or something, easy to break down. Depending on lighting will depend on if you find them transparent. I don't mind possible vague outlines; I was looking so no real details of my belongings in the background could be seen (shelves; figurines; etc.) My apt living room has no overhead light source, so floor lamps give light. In the 3 photos of 2 of the screens put together; the first is with a lamp in front and back of the screen as well as a tv on. The next has the tv off lamps still on and the screen reflecting the pc monitors. The third is with both lamps and the tv off, monitors on but not as reflective.
In the box, you have all the parts you need as well as a small tool and an instruction manual. Item 'bags' are number [though they may come off in the box] to assist with assembly. Now assembly can be easy or hard depending on your abilities and the area you are working in. I have bad knees, so kneeling was not an option for me. My first attempt to put this together, I put the 'feet bases' on while still connecting the top bar. It made it twice as hard, and I stretched the fabric more than was necessary causing wholes around some of the stitching and stretching them a little. As well as causing some stitching to come undone on one of the pictured areas. Once I took off the 'feet bases', I had an easier time to screwing in the top bars. You will still need some hand strength, which I don't have much of, but it is possible. I use the grip things to open pickles jars.
Once the screens were assembled, I followed the instructions of tying an outer screen to the inner screen and using the Velcro 'privacy flaps' to complete the connection. I have what I think is low shag carpeting which they were able to stand stably on once I wiggled them into standing straight up and in place.
Given these post pandemic times, I am overall happy with both the divider and the price. The 2 things I do not like about the divider is the difficulty putting in the top bar [after connecting the top and sides] and storability when not in use issue I am having. Others with larger space will likely not have the last issue.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 19, 2025
★★★★★ 5
100% worth it - I recommend to EVERYONE
I've seen coworkers and instructors using folding screens behind them for video calls since the pandemic started, and I finally gave in and purchased this one. I freaking LOVE it. I love that I can be on a video call and not have to worry about the angle of my laptop or having people 'in' my home while I'm at work. It's so nice. I may eventually buy and deconstruct a wall hanging to create some colorful fabric panels instead of the brown, but (and I say this as NOT a brown fabric-lover) the brown looks pretty nice. It's a very dark brown, and it serves as completely neutral while still feeling a bit warm.
- No need for tools to assemble, even though it says screwdriver.
- One thing: I thought it would fold up flat, and it does NOT fold up flat. Important to know. I just keep it folded up into a 16.5" (grabbed a tape measure to check) square column by my desk and unfold whenever I have video conferences.
If you can't tell, I really like having this thing around. 100% recommend and would buy again.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 24, 2021
★★★★★ 4
it's a utility screen
Color: Black, Size: 3-Panel
It's a basic, utilitarian screen. It's what I expected it to be. Assembly isn't hard, but it does require two people when stretching the fabric between the poles. I got it to use in a closet to separate the water heater from the washer/dryer, so it'll be perfect for that. Pros: it's opaque and tall (my 6'4" husband can't see over it unless he is standing against it) and I like the ability for the three pieces to stand separately of each other. Cons: it's flimsy. It wobbles and will tip over easily.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 9, 2022
★★★★★ 5
Great for the price
The item is great bit I wish it were a bit more sturdy and it folded flat. It folds into a square so it does take up some room when it's not in use.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 17, 2021
★★★★★ 1
Cheap, screw holes don't line up, screen is to short, and bars take WD-40 and a mallet
This has been my worst purchase on Amazon ever. DO NOT BUY. My first issue was that bars #4 and #5 would not go together, I ended up buying WD-40 and a mallet and that kind of got them together. Now I went to but the screen on (which is almost see thought) and the screen is to short. I can't attached the bottom bar to the tall bar. The screw hole is in the wrong spot, so the screen is not secure. No amount of pulling on the screen will make it grown .25" longer. Since I can't get the bars secure the walls are not steady and fall over easily. The feet did go together very nice. I can't return them according to Amazons policy. I bought 2 sets. I'm not going to try to build the other set I have. And I can't take apart the first set, since the bars are so tightly together. Also the polls have rust on them.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 10, 2023
recommand products
엔시티127 | NCT 127 [ STICKER ] POSTCARD + HOLOGRAM PHOTO CARD SET
14.99
아이즈원 | IZ*ONE 1ST JAPANESE ALBUM [ TWELVE ] A VERSION
54.99
비원에이포 | B1A4 SPECIAL DVD [ 꽃보다 B1A4 ] DVD + PHOTOBOOK
99.99
있지 | ITZY 1ST ALBUM [ CRAZY IN LOVE ] SPECIAL EDITION JEWEL CASE VERSION
19.99
P1HARMONY OFFICIAL LIGHT STICK
59.99