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Description
Human TFPI2 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample handling and requirements: Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge at 1000g for 15 minutes at 2 8C within 30 minutes of collection. Remove the supernatant for testing or store at
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample handling and requirements: Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge at 1000×g for 15 minutes at 2-8°C within 30 minutes of collection. Remove the supernatant for testing or store at -20°C or -80°C, but avoid repeated freezing and thawing. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare a gradient standard working solution: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Tissue Factor Pathway Inhibitor 2 (TFPI2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Tissue Factor Pathway Inhibitor 2 (TFPI2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Tissue Factor Pathway Inhibitor 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Tissue factor pathway inhibitor 2 (TFPI-2) is a member of the Kunitz family of serine protease inhibitors and a structural homolog of tissue factor pathway inhibitor (TFPI). TFPI-2 inhibits the tissue factor/factor VIIa (TF/VIIa) complex and multiple serine proteases, including plasminogen activators, plasminogen activators, factor XIa, trypsin, and chymotrypsin. TFPI-2 functions to maintain tumor milieu stability, inhibiting tumor invasiveness and growth, as well as metastasis formation. It also induces apoptosis and inhibits angiogenesis, which may significantly contribute to tumor growth inhibition. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | plasma |
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4.3 ★★★★★
Based on 10 reviews
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Product Reviews
★★★★★ 5
Super Cute, Fun, & Effective
Color: Lightgreen
This set of water flosser and electric toothbrush are just adorable! The shapes of them fit in the hands really comfortably, but also add to the cuteness as the shapes of the animals!
Yes, this is meant for kids - so the toothbrush heads are small (for little kids), and the water flosser doesn't spray terribly hard. BUT they're still very effective.
I especially like the water flosser. It can be taken into the bathtub/shower while bathing, so the spray doesn't end up going places we don't want water to go. It has a small tank of water, and I recommend using room temp water (rather than cold) so that it's comfortable on the gums and teeth. Also to note, the flosser doesn't work when it's sideways or upside-down - it needs to be held upright.
This set is so cute, easy to use, easy to re-charge, and very comfortable. It's also very good quality - a great purchase. I love it!
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Reviewed in the United States on March 17, 2026
★★★★★ 4
4 year old approved!
Color: Lightgreen, Color: Lightgreen
Love that this is a complete set, down to the wall block for the charger!
My daughter is 4 and absolutely loves this set. I personally feel like the toothbrush head is a tad small, but it does do the job well. Its a soft brush head, with plenty of replacement heads. The toothbrush and the flosser both arrived fully charged, and so far we havent had to recharge it, but we're only a few days in. So, it seems to have a pretty good battery life so far. (I will update my review if anything changes on that aspect)
The toothbrush and flosser both stand upright independently on the counter, without tipping over.
The water flosser is hard fir my daughter to do by herself. She sprays water everywhere, as theres several buttons on it and she cant seem to remember in time which button turns it off. I help her with flossing and its been going fine.
Whether youre on high or low the water pressure is not too hard for a kids mouth. In fact, I think the low setting does nothing but rinse the mouth and basically teach the habit of flossing. We use the high setting and it does and OK job. I am a little old school though, and feel like nothing will top a good ole dental floss flossing, but water flossing is a safer route to avoid cutting gums or getting floss stuck between close teeth.
The sound level is minimal, a fraction of the sound level my adult water flosser makes. It doesnt shake/vibrate too badly in the hand and theyre bith comfortable to hold, and size appropriate for my (4yrs) daughters hands.
It comes in a really nice box, packaged perfectly, making this a great gift for your little ones. The colors and theme makes brushing teeth exciting and fun again for your littles :)
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Reviewed in the United States on October 25, 2025
★★★★★ 5
Great for kids
Color: Lightgreen
The HUACOCARE Kids Water Flosser and Electric Toothbrush Combo is an absolute game-changer for establishing a solid dental hygiene routine with children. The light green giraffe and monkey design is incredibly charming and immediately turned what used to be a bedtime struggle into a fun activity that my child actually looks forward to. Having both the sonic toothbrush and the water flosser in one compact unit is brilliant, as it saves counter space while ensuring that every part of their mouth gets a professional-level clean. The three gentle flossing modes and two sonic brushing modes are perfectly calibrated for younger, more sensitive gums, providing a thorough clean without any discomfort.
One of the standout features of this set is how braces-friendly it is; the water flosser reaches those tricky spots around wires and brackets that a standard toothbrush simply can't touch. It is clear that a lot of thought went into the ergonomics and the "fun factor," making it a perfect gift that is both practical and engaging. Since we started using this combo, our dentist has noticed a significant improvement in plaque removal and overall gum health. If you are looking for an all-in-one solution to build healthy brushing habits and make dental care feel like less of a chore, this set is worth every penny.
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Reviewed in the United States on February 21, 2026
★★★★★ 5
smells and tastes like strawberries. good for encouraging wanting to brush teeth!
Style: Strawberry Fluoride-Free
my toddler LOVES this toothpaste. the taste is great so it encourages wanting to brush teeth and the smell is really good too. every time we go to the bathroom, my toddler thinks it’s time to brush his teeth. i've had no problems with this toothpaste as it works really well getting the job done.
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Reviewed in the United States on May 8, 2026
★★★★★ 5
Sweet Marshmallowy Heavens.
My kid loves this. It's all he uses. He does manage to get it all over the world. I'm not even sure how. It's like... coming out of his hair, rolling down my cabinets, wandering into the kitchen. But that's not the fault of Burst. At least not until they make disappearing toothpaste. Disintegrating toothpaste! OMG I NEED THAT. Can we get toothpaste that disappears after the 8 year old runs wild with it? Please? In lieu of disintegrating toothpaste... this is the bomb. He can't stand the minty stuff and says everything is "TOO SPICY." Welcome to life kid. It's all too spicy out here. Except this toothpaste. It's perfect.
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Reviewed in the United States on March 14, 2026