SKU: 44867423531

Mouse IL-10 ELISA Kit

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Description

Mouse IL-10 ELISA KitProduct Specification Description Detection Principle: This kit uses double antibody sandwich enzyme linked immunosorbent assay technology. The specific anti mouse il 10antibody was pre coated on a high affinity microplate. The standard, sample to be tested and biotinylated detection antibody are added into the wells of the enzyme plate. After incubation, the il 10present in the sample combines with the solid phase antibody and detection antibody to

Product Specification

Description

Detection Principle:

This kit uses double antibody sandwich enzyme-linked immunosorbent assay technology. The specific anti mouse il-10antibody was pre coated on a high affinity microplate. The standard, sample to be tested and biotinylated detection antibody are added into the wells of the enzyme plate. After incubation, the il-10present in the sample combines with the solid-phase antibody and detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase labeled streptavidin (streptavidin HRP) was added. After washing, the chromogenic substrate was added to avoid light for color development. Stop the reaction by adding stop solution, and determine the absorbance value at 450 nmwavelength (reference correction wavelength 540nmor 570nm)

Detection Type: double antibody sandwich method

Form: pre coated 96orifice plate

Detection Sample Type: cell supernatant, serum, Plasma

Loading amount: 100ul

Kit Components:

Pre coated 96well plates, standards, anti mouse il-10detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrpA copy of sealing film and instructions.

Sensitivity: 1.8 pg/ml

Detection Range: 31.2 - 2000 pg/ml

Recovery Range: 95-103%

Storage Method: 2-8℃

Background:

Mouse interleukin -10 (IL-10) also known as cytokine synthesis inhibitor (CSIF). It is IL-10 & Alpha-a member of the helical cytokine family, which also includes il-19, il-12, il-22, IL-24 , and il-26/ak155. Many activated hematopoietic stem cells, hepatic stellate cells, keratinocytes, and placental cytotrophoblasts secrete il-10. Human IL-10 is active in mouse cells, but mouse IL-10 does not act on human cells. The amino acid homology of mature mouse IL-10 and rat IL-10 is 85%, and that of cattle, dogs, horses, cats, humans, sheep, and pigs is 70~77%. Mature mouse IL-10 is a noncovalently bound homodimer with a molecular weight of 36 kDa containing two intrachain disulfide bonds.

IL-10 by the II type cytokine receptor IL-10 r& Alphaand IL-10 & amp; Betathe heteromeric receptor complex that is composed of exerts its biological activityIL-10 r& Alphais a 110 kDa transmembrane glycoprotein expressed in lymphocytes, natural killer cells, macrophages, monocytes, astrocytes, intestinal epithelial cells, cytotrophoblasts, and activated hepatic stellate cells, while 75 kDa il-10r& Beta

IL-10 is involved in immune regulation of inhibitory and activating effectsIL-10 as an anti-inflammatory cytokine, promotes the formation of M2 macrophages by inhibiting the activation and expansion of Th1 cells and Th17 cells. Immunosuppression regulates the expression of T cells and B cells, which play an important role in Treg cell proliferation (19) . In the tumor microenvironment, IL-10 inhibition regulates the proliferation of T cells and myeloid derived suppressor cellsIL-10 induces the accumulation of intratumoral material and activates cd8+ T cellsIL-10 plays an important protective role in limiting tissue damage in joint inflammation and promoting muscle regrowth after injury, but also contributes to persistent viral infection. IL-10 is elevated in Sjogren's syndrome (saliva) and primary central nervous system lymphoma.

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SKU: 44867423531

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Rhianna Sullivan
Chelsea, US
★★★★★ 4
No big surprises but it'll put you through the gamut.
Format: Kindle
What a weirdly heartfelt, grotesquery of a tale. I haven't had a horror novel drag me through this much emotional goo in ages. Hating characters. Suddenly pitying them. The schadenfraude of watching parts of the story unfold. And all the while just loathing the puppets... I've always had mixed feelings about them (if it weren't for the saintly Mr. Roger's Neighborhood I'm certain it'd be pure hatred). If you like your horror with gore and chaos and a healthy dose of w-tee-eff-ery you're sure to find this one right up your alley. It dragged at parts and more than once I was sure it should have reached it's natural conclusion without what felt like there-for-the-lolz filler. All that said it may have been because I had figured out the conclusion and ending very early and every confirming detail made me ready to get there. Still enjoyable because Hendrix wrote it so well but I did find it predictable. Not a favorite but one I'd recommend to comedy horror lovers for sure.
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Reviewed in the United States on February 1, 2026
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Chopinfan78
Cuba, US
★★★★★ 3
Why is it 400+ pages?
Format: Hardcover
I haven’t disliked a book this much in a very long time. The characters are unlikeable, the puppets are annoying, the child is annoying, and the reason for the family secret (though I understand grief can sometimes make us do weird things) was (for me) unremarkable. For the first 75% of the book it seems like we’ve been lied to and the book isn’t about ghosts at all, that it's about a creepy little puppet possessed by a poltergeist. But then we learn the house and all the creepy dolls, puppets, and taxidermied animals are, in fact, haunted. I won’t tell you by what/who because that will spoil “the twist”. What I will tell you is that it is an unremarkable twist is a story that is too long. The only real enjoyment I got out of this book was when I read the last page. So, why have I given it 3 stars? Because it isn’t a bad book. It just isn’t for me. I wanted a clear ghost story that didn’t involve creepy puppets and uncompelling characters. Instead, I got Pupkin and Louise and Mark. In fairness, though, Mark actually develops over the course of the story. If you don’t mind annoying puppets seemingly possessed by a poltergeist (wink, wink), an MC who continues to deny what’s happening until denial is no longer an option (yes, I know that was the point of that section), family secrets that make no sense, and character conflict that could be easily resolved by just talking to one another, then you’ll probably enjoy this book.
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Reviewed in the United States on November 24, 2024
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lianne steffenson
Phoenix, US
★★★★★ 5
Love it!
Great product! Excellent coverage! Will definitely purchase again
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Reviewed in the United States on June 2, 2026
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Kathleen Jarboe
Battle Creek, US
★★★★★ 5
Sunscreen
Lightweight and gives great protection
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Reviewed in the United States on May 17, 2026
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Dupont Els
Lowell, US
★★★★★ 5
Love it
Very happy with this product
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Reviewed in the United States on April 22, 2026

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