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Description
EdU-555 cell proliferation assay kitProduct Specification Usage Before the trial guidelines 1, the corresponding animal experiment, EdU suggested initial dosage of 50 mg kg (50 mu g g), dilute concentration is 0. 5 1 mg mL. Are injected into mice weight 20 g, mg, 1 to 1 mg mL, need injection of 1 mL. 2, for the cells, the recommended 10 microns, EdU work for in vitro culture cell, specific EdU use concentration, incubation time along with the samples and research purpose is different,
Product Specification
| Usage | Before the trial guidelines 1, the corresponding animal experiment, EdU suggested initial dosage of 50 mg/kg (50 mu g/g), dilute concentration is 0.5 1 mg/mL. Are injected into mice weight 20 g, mg, 1 to 1 mg/mL, need injection of 1 mL. 2, for the cells, the recommended 10 microns, EdU work for in vitro culture cell, specific EdU use concentration, incubation time along with the samples and research purpose is different, can be adjusted appropriately. 3, this kit can be carried out about 200 t of 6 orifice EdU experiments, about 2000 t96 orifice or paraffin section EdU experiment, experiment should be calculated according to the sample situation in the process of usage. I. Sample processing 1, the animal experiment (1) EdU injection in animals In mice, can according to the amount of 10-200 mg/kg, the EdU with PBS mixed with a certain concentration, intraperitoneal injection, specific tissues or organs local injections or added to drinking water. The specific dosage is related to the type, weight and mode of use of animals, and can be referred to the relevant literature. Therefore, it is recommended to conduct a certain exploration of the use concentration of EdU for the first use, or directly use the concentration of 50mg/kg for testing. Injection method: the experiment according to the customer, such as abdominal cavity injection, subcutaneous injection, intramuscular, tail intravenous injection, etc., among them with intraperitoneal injection. After 6 h or after the appropriate time was determined according to the specific experiment, the mice were quickly sacrificed, the desired tissue was removed, and frozen or paraffin sections were made according to routine procedures. EdU labeling can also be adjusted by referring to the relevant literature. Small intestinal epithelial cells proliferate rapidly, and positive information can be detected 6 hours after EdU injection in adult mice, which can be used as a positive control for pre-experiments. (2) Slice processing Pre-section treatment: It is best for tissues and organs to be washed to remove residual EdU in blood and tissues and reduce background. Section thickness: 3-10μm is appropriate, too thick section may affect the section background and staining efficiency. Slice post-processing: Paraffin sections were deparaffinized twice with xylene for 15 min, once each with gradient ethanol (100%, 95%, 85%, 75%) for 5 min, and once with deionized water for elution. Frozen section processing: room temperature placed after 30 minutes, fixed 10 minutes, PBS cleaning three times, each time for 5 minutes. 2. Cell experiments (1) cells EdU markers a.with cell complete medium and EdU mother liquor by 2000:1 the ratio of dilution, preparation of a moderate amount of 10 microns EdU medium; b.each hole adding suitable amount of 10 microns EdU medium 2 hours incubation, abandon culture medium (optimal incubation time for 1/10 of the cell cycle) commonly, EdU incubation medium and EdU reaction liquid volume may refer to the table below; c.PBS cleaning cell 3 times, each time for 5 minutes.
a. Cell fixative (PBS containing 4% paraformaldehyde) was added to each well and incubated at room temperature for 15 minutes. The fixative was discarded and washed with PBS for 3 times, 5 minutes each time. b. Each well was decolorized with an osmotic agent (0.5% TritonX-100 in PBS) and incubated for 15 minutes. PBS was washed three times for 5 min each. Second, EdU response
The EdU reaction solution was prepared according to the ratio of PB: CU: AC: 555 chromogen = 855:40:100:5 (the reaction solution was prepared immediately). Each sample add 50-500 mu L EdU staining reaction liquid liquid evenly cover samples (reaction), 15 to 60 minutes at room temperature away from light incubation, abandon the dyeing liquid reaction, PBS wash 3 times, each time for 5 minutes. 3. DAPI staining DAPI(100x) was diluted with PBS at a ratio of 1:100 to make ready-to-use DAPI staining solution. 50-500μL of DAPI staining solution was added to each sample and stained in the dark for 15 minutes, followed by three washes with PBS for 5 minutes each. 4. Take pictures Fluorescence microscope, confocal microscope or whole-film scanner were used to collect images. After staining, the slides were stored in a dark place at 4℃. Above are stained images of 50mg/kg labeled mice for 6 hours |
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| Description | EdU(5-ethynyl-2' -deoxyuridine), also known as 5-ethynyl-2' -deoxyuridine (5-ethynyl-2' -deoxyuridine), is a thymidine analogue with an alkynyl hydroxyl group that is rare in natural compounds and can replace thymidine (thymidine, 5-ethynyl-2' -deoxyuridine) in cell proliferation. thymidine (thymidine) is inserted into the replicating DNA molecule, the ethynyl group on EdU can react covalently with the fluorescently labeled small molecule Azide probe (Azide Alexa Fluor 488, etc.) through the catalysis of copper monovalent ion to form a stable triazole ring. "It is called the Clickreaction, which allows for an efficient and rapid measurement of cell proliferation, especially the percentage of cells in S phase." With traditional immunofluorescence staining (BrdU) method compared with EdU only 1/500 the size of the BrdU antibody, are more likely to spread within the cell, don't need strict sample degeneration (acid solution, pyrolysis, enzyme solution) processing, effectively avoid the damage samples, help in the overall level of tissue and organ observation of cell proliferation, It has higher sensitivity and faster detection speed.Note: AC is a powder, need to add 10mL pure water before use, fully dissolved before use, AC is easy to oxidation failure, after the refrigerator is stored at -20 degrees, if found discoloration, need to replace fresh reagent. In animal experiments, the mass concentration of 100mM EdU mother liquor corresponds to 25mg/mL, which should be converted according to the dosage.
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| Storage Temp. | -20 ° C, valid for 12 months. |
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4.8 ★★★★★
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★★★★★ 4
Soft blanket
Size: California King, Color: Gray
This soft blanket is nice and warm
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Reviewed in the United States on September 14, 2025
★★★★★ 5
Keep em soft and lasting!
Size: Queen, Color: Dark Gray
Oh wow are these things soft. I bought them for my RV and Toy Hauler beds and they feel terrific. They are warm and probably won't come apart on you if you take care of them. The trick to that for me seems to be not to let them get "Crunchy". If you've owned these before you know what I mean. When the backing material begins to go and they fall apart in tiny chunks.
This pro-tip is how mine have lasted longer than I had hoped:
1. Wash them in cold water. Hot water tends to get to these things after a few washed. Very little agitation as well. Don't spin them round and round and rip them up. Wash on the gentle cycle.
2. No bleach, color safe or not, and try to use very little detergent or use something like Woolite for delicates. Baby clothes detergent also works well. Something super mild.
3. Dry them with no or very little heat on a very low tumble. I tend to do the two I have together and that's all I put in the dryer.
Mine have held up amazingly well for the abuse they take and they are a fluffy as new. This is a win product for the price, you won't regret it if you take care of it.
Hope you found this helpful!
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Reviewed in the United States on April 26, 2024
★★★★★ 5
Just what I was looking for.
Size: California King, Color: Ivory
Loved the color and softness. Washed and dryed upon receipt. No shrinkage and no lint in the lint trap.
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Reviewed in the United States on March 19, 2026
★★★★★ 5
Love the color, and texture of the blanket. Great price too!
Size: California King, Color: Peacock Blue
We have this blanket sandwiched between our top sheet and comforter. My husband feels nice and warm with the blanket, but I'm a warm sleeper. I feel comfortable also since a keep a floor fan aimed to my side of the bed. Problem solved and we both get a comfortable, good night's sleep.
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Reviewed in the United States on October 26, 2025
★★★★★ 5
Soft, Luxurious & Perfect for Hair and Skin!
Size: Queen (20" X 30"), Color: 11 - Light Turquoise, Number of Items: 2, Size: Queen (20" X 30"), Color: 11 - Light Turquoise, Number of Items: 2
These satin pillowcases are honestly amazing. The material feels super soft, smooth, and cooling on the skin, and they make my bed look so elegant and luxurious. I bought them mainly to help protect my hair from frizz and dryness, They’re also very comfortable to sleep on and don’t feel cheap at all. The color is beautiful in person, exactly like the photos on Amazon. I washed them already and they still kept their softness and shine. Definitely worth the purchase for the price I would 100% buy again and recommend to anyone looking for cute, comfortable satin pillowcases.”
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Reviewed in the United States on May 13, 2026