SKU: 68633454994

UltraNuclease

Sale price$75.60 Regular price$84.00
Save 10%

Pay in installments of $21.00 with ShopPay, AfterPay and Klarna

Shipping Estimate
USA
  • USA
  • CAN

Ships within 48 hours · Estimated delivery Jul 22 - Jul 27

Promo Codes Available:

For Your Every Summer RSVP, with Code: SUMMER15

Description

UltraNucleaseProduct Specification Species Serratia marcescens Synonyms SMNE,Nuclease, UltraNuclease, Benzonase Amino Acid Sequence Asp22 Asn266 DTLESIDNCAVGCPTGGSSNVSIVRHAYTLNNNSTTKFANWVAYHITKDTPASGKTRNWKTDPALNPADTLAPADYTGANAALKVDRGHQAPLASLAGVSDWESLNYLSNITPQKSDLNQGAWARLEDQERKLIDRADISSVYTVTGPLYERDMGKLPGTQKAHTIPSAYWKVIFINNSPAVNHYAAFLFDQNTPKGADFCQFRVTVDEIEKRTGLIIWAGLPDDVQASLKSKPGVLPELMGCKN Expression System E. coli Molecular Weight 27. 7kDa (Reducing) Purity 95% by

Product Specification


Species Serratia marcescens
Synonyms SMNE,Nuclease, UltraNuclease, Benzonase
Amino Acid Sequence

Asp22-Asn266

DTLESIDNCAVGCPTGGSSNVSIVRHAYTLNNNSTTKFANWVAYHITKDTPASGKTRNWKTDPALNPADTLAPADYTGANAALKVDRGHQAPLASLAGVSDWESLNYLSNITPQKSDLNQGAWARLEDQERKLIDRADISSVYTVTGPLYERDMGKLPGTQKAHTIPSAYWKVIFINNSPAVNHYAAFLFDQNTPKGADFCQFRVTVDEIEKRTGLIIWAGLPDDVQASLKSKPGVLPELMGCKN

Expression System E.coli
Molecular Weight

27.7kDa (Reducing)

Purity

>95% by SDS-PAGE

Conjugation Unconjugated
Tag His Tag
Physical Appearance Liquid
Storage Buffer

10mM Tris (pH7.4), 500mM NaCl, 2mM MgCl2, 50% glycerol

Stability & Storage

· 12 months from date of receipt, -20 to -70 °C as supplied. 
· 1 week, 2 to 8 °C under sterile conditions after reconstitution.  
· Please avoid repeated freeze-thaw cycles.

Reference

1.Nestle M, Roberts W K. An Extracellular Nuclease from Serratia marcescens I. PURIFICATION AND SOME PROPERTIES OF THE ENZYME[J]. Journal of Biological Chemistry, 1969, 244.

2.Kim W Y, Lee H S, Suh S J, et al. Purification and Cellular Localization of Extracellular Nuclease of Serratia marcescens Expressed in Escherichia coli[J]. Korean Journal of Microbiology, 1994, 32(2):147-154.

Background

Multi Nuclease all-around nuclease, also called broad-spectrum nucleic acid enzyme, is a kind of comes from Serratia Marcescens restriction endonuclease. It is capable of degradation of all forms of DNA and RNA (double-stranded, single-stranded, linear, circular or superhelical forms) under a very wide range of conditions (6Murea, 0.1M GuanidineHCl, 0.4%TritonX100, 0.1%SDS, 1mM EDTA, 1mM PMSF). The formation of 3-5 oligonucleotide residues containing 5 '-phosphate terminus is widely used to remove nucleic acids from biological products. The expression and purification of this product in Escherichia coli(E.coli) through genetic engineering can not only reduce the viscosity of cell supernatant and cell lysate in scientific research, but also improve the efficiency of protein purification and functional research. It can also be used in virus purification, vaccine production, protein and polysaccharide pharmaceutical industry as a host residual nucleic acid removal reagent, reducing the host residual nucleic acid to the peak (pg) level to improve the efficacy and safety of biological products. And can effectively prevent human peripheral blood monocyte (PBMC) clumping in cell therapy and vaccine research.

Components

Components

Amount

UltraNuclease *

250U/μL

Buffer Formulation

10mM Tris (pH7.4), 500mM NaCl, 2mM MgCl2, 50% glycerol

* One unit of Nuclease is defined as the amount of enzyme that causes a ∆A260 of 1.0 (equivalent to the complete digestion of 37μg DNA) in 30min.

Protocol

1.Sample preparation:

Adherent cells: Remove the medium, clean the cells with PBS, and remove the supernatant.

Suspension cells: Cells were collected by centrifugation, cleaned with PBS, centrifuged at 6,000rpm for 10min, and precipitates were collected.

Escherichia coli: The bacteria were collected by centrifugation, cleaned once with PBS, centrifuged at 8,000rpm for 5min, and precipitates were collected.

2.Sample treatment:

The collected cell precipitates are cleaved according to the ratio of mass (g) to volume (mL) to 1: (10~20). Cells can also be cleaved mechanically or chemically on ice or at room temperature (1g cells are about 109).

3.Enzyme addition:

the proportion of 1g cell precipitation digested by 250Units is required. You can also choose the addition plan according to the recommended dosage in the table above, increase the amount of enzyme within a certain range, and reduce the digestion time accordingly.

4.Supernatant acquisition:

The supernatant of cell lysis solution was obtained by centrifugation at 12,000rpm for 30min, and then subsequent related experiments were conducted.

Conditional parameter

Optimum condition

Applicable condition

Mg2+

1-2mM

1-10mM

PH

8.0

6-10

Temperature

37℃

0-42℃

DTT

0-100mM

>0mM

β-Me

0-100mM

>0mM

Monovalent cation

0-20mM

0-150mM

phosphate anion

0-10mM

0-100mM

Unit Definition

One unit of Nuclease is defined as the amount of enzyme that causes a ∆A260 of 1.0 (equivalent to the complete digestion of 37μg DNA) in 30min

Shipping Notes
  • Free Standard Shipping on $100+ Orders to the USA.
  • Except Preorder products are shipped in 48 hours.
  • Delivery to the USA:
  1. Standard Shipping : 3-10 business days
  • If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
  • Final sale items are not eligible for returns or exchanges.
  • To process your return/exchange, please contact us at [email protected]
  • Please click here for more details>>> Return & Exchange Policy
SKU: 68633454994

Discover Niche Categories That Outsell

Top-Converting Item to Boost Your Average Order

4.5 ★★★★★
Based on 29 reviews
Sort
Highest Rating
Newest First
Oldest First
Product Reviews
J
Verified Purchase
Juan Carlos Brenes
Lowell, US
★★★★★ 5
Filtro de aceite pentastar
Color: 3 Set
Perfecto para enfriadores de aceite pentastar V6 2018
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 15, 2026
G
Verified Purchase
G. Richardson
Omaha, US
★★★★★ 5
Good price and appears exactly like OEM
Color: 3 Set
Good price for set of 3 also includes o-rings for cap. After finding engine litter on a rocker lifter change wanted to run several filters in quick succession changing the filter every 100 miles and topping off the oil. seems to be working well and catching any glitter.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 6, 2026
E
Verified Purchase
Eli Korcz
Alexandria, US
★★★★★ 1
Doesn't fit eco diesel
Color: 1 Set
Says fits ram eco diesel but it doesn't.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 20, 2025
A
Verified Purchase
Amazon Customer
Los Angeles, US
★★★★★ 5
Effective
Size: 300 Milliliter
This worked wonders on my car’s performance! After one use, the engine started idling much smoother and the throttle feels more responsive. A very high-quality German-made product.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 24, 2026
M
Verified Purchase
Matt
Port Orchard, US
★★★★★ 5
Great for All Cars but Especially German Cars
Size: 300 Milliliter
I use this in our used VW we bought and it helps keep the carbon build up down with their direct injection feature. I pair it with their oil additive which helps lubricate the rings so they don't stick as much.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 22, 2026

recommand products