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Description
Human TNF-α ELISA KitProduct Specification Species Reactivity Human Theory This kit adopts double antibody sandwich enzyme linked immunosorbent detection technology. Specific anti human TNF antibodies were pre coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, TNF present in the sample binds to the solid phase antibody and the
Product Specification
| Species Reactivity | Human | |||||||||||||||||||||||||||
| Theory | This kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human TNF-α antibodies were pre-coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, TNF-α present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm). | |||||||||||||||||||||||||||
| Synonym | APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, tnfa, tnf-a, TNFalpha, TNF-alpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor, tumor necrosis factor-alpha | |||||||||||||||||||||||||||
| Composition |
Please use it within the validity period of the kit (new and old products are shipped randomly)
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| Background | Human TNF-α is a 26kDa type II transmembrane protein consisting of a 35 amino acid (aa) intracellular domain, a 21aa transmembrane segment, and a 177aa extracellular domain (ECD). In the ECD region, human TNF-α has 97% amino acid sequence homology with macaque monkey, and 71-92% amino acid sequence homology with cattle, dog, cotton rat, horse, cat, mouse, pig and rat. It can be expressed by a variety of different cells such as immune cells, epithelial cells, endothelial cells, tumor cells. TNF-α can induce lysis of tumor cells and virus-infected cells, and binds to soluble TNFRI to generate transmembrane transfer signals of downstream cells. TACE/ADAM17 can cause the shedding of TNF-α-containing cell membrane to release active TNF-α cytokine. It is a trimer composed of TNF-α extracellular soluble structure with a molecular weight of 55 kDa. TNF-α has two receptors: TNFRI and TNFRII. TNFRI has a molecular weight of 55-60kDa and is widely expressed; TNFRII has a molecular weight of 78-80 kDa and is limited to hematopoietic cell expression. Both are expressed as homotrimers; TNF-α and TNFRI and TNFRII bind with similar affinities and can promote the activation of NFκB. However, only TNFRI has a cell death domain, which can trigger apoptosis. Both soluble receptors are released into human serum and urine and can neutralize the activity of TNF-α. |
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| General Notes | 1. Please use the kit within the validity period. 2. The components of different kits and different batch kits cannot be mixed. 3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step. 4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc. 5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it. |
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| Storage Temp. | Kit unopened, stored at 2-8 °C. | |||||||||||||||||||||||||||
| Test Range | 15.6pg/mL-1000pg/mL |
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4.8 ★★★★★
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Product Reviews
★★★★★ 5
A Book for our Times
Format: Kindle
This book has excellent advice for all Christians, as well as those not sure of what the Bible says. The passages, illustrations, scriptures are presented logically, Biblically, and hold much wisdom. I enjoyed the encouragement, practical steps that all can use.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 15, 2024
★★★★★ 5
The World of the End book
Format: Paperback
It's very good book written by Dr. David Jeremiah
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Reviewed in the United States on November 7, 2025
★★★★★ 5
Diverse Worship--Make A Joyful Noise Unto The Lord All Ye Lands
Format: Hardcover
I love this beautifully illustrated story. Children and adults, too, learn God's vision for His church—true worship. Author Dorena Williamson presents an image of a church no longer divided by race, culture, or style of worship. Instead, the church is a gathering of God's people celebrating who He is and all He has done. Also included are author notes suggesting ways to share the importance of embracing and celebrating God's diverse world with children.
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Reviewed in the United States on November 10, 2021
★★★★★ 5
We Are Better Together
Format: Hardcover
One Sunday morning when all the Sunday school classes landed in a heap for a multi-generational assembly followed by muffins, I read aloud from The Celebration Place, Dorena Williamson’s anthem to the Body of Christ gathered. How refreshing to share the truth that worship (and worshipers!) will look and participate differently in other places!
Here in rural Maine where a choir is as rare a sight as a brown face, Erin Bennett Banks’s vivid illustrations enriched the text, and I was pleased to hear adult chuckles accompanying the page about rappers “nodding to the beat” while “old folks stomp their feet.” Together, author and illustrator announce the church as “no longer a divided space. Now it’s a celebration place!”
With so many fractures in our world upon which we can distance and divide, the pursuit of unity in the Body of Christ is difficult but crucial. The work toward delightful diversity done today in God’s House is affirmation of the truth that we are better together, and it is preparation for the day when we will join with all nations God has made to glorify his name, for he is great and worthy of our celebration.
Many thanks to IVP Kids for providing a copy of this book to facilitate my review, which is, of course, offered freely and with honesty.
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Reviewed in the United States on December 6, 2021
★★★★★ 5
A message of unity
Format: Hardcover
The Celebration Place is a vibrant picture book highlighting diverse cultures and people worshipping God in a variety of glorious ways. This inclusive book portrays vocal artists, indigenous groups, and a person in a wheelchair in praise of a God whose presence reaches around the globe. The book’s message rings clear: when God’s people assemble in love, the church is a celebratory place. The back matter prompts further conversation. Children and their caregivers will want this important book in their hands and on their shelves. This honest review is based on an e-advanced reader copy from the author and publisher.
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Reviewed in the United States on November 9, 2021