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Description
RFX5 Recombinant Rabbit mAb (S-3628-50)Product Specification Host Rabbit Antigen RFX5 Synonyms DNA binding protein RFX5; Regulatory factor X 5 Immunogen Synthetic Peptide Location Nucleus Accession P48382 Clone Number S 3628 50 Antibody Type Recombinant mAb Isotype IgG Application WB, IHC P Reactivity Hu Positive Sample HeLa, Jurkat, K562, Raji, HCT 116 Purification Protein A Concentration 0. 5 mg ml Conjugation Unconjugated Physical Appearance Liquid Storage Buffer PBS, 40% Glycerol, 0.
Product Specification
| Host | Rabbit |
| Antigen | RFX5 |
| Synonyms | DNA-binding protein RFX5; Regulatory factor X 5 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P48382 |
| Clone Number | S-3628-50 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | HeLa, Jurkat, K562, Raji, HCT 116 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:500 | Hu |
Background
The RFX5 protein is a core subunit of a key transcription factor complex regulating adaptive immunity; its canonical function involves assembling with RFXAP and RFXANK into a heterotetramer (an RFX5 dimer bound to one RFXAP and one RFXANK molecule), recognizing the X-box motifs in MHC-I and MHC-II promoters via its N-terminal conserved domain, and utilizing its C-terminal domain to collaborate with NF-Y and other factors in stabilizing transcriptional enhanceosomes—deficiencies in this function lead to primary immunodeficiencies such as bare lymphocyte syndrome (BLS). The latest research revealed at the structural level that RFX5 also acts as a unique pioneer transcription factor: its extended DNA-binding domain (eDBD) directly forms hydrogen bonds with histones via the Glu102 residue, specifically targeting the SHL+2 position on nucleosomes. This induces localized DNA distortion and the dissociation of distal DNA from the nucleosome surface, thereby actively opening chromatin to enhance DNA accessibility and paving the way for subsequent transcription factor binding.
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