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Description
Mouse CTTN ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with cortactin (CTTN) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of cortactin (CTTN) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Cortactin ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Cortactin (derived from "cortical actin-binding protein") is a monomeric protein located in the cytoplasm that is activated by external stimuli to promote polymerization and rearrangement of the actin cytoskeleton, particularly at the cell periphery. It is present in all cell types. Upon activation, it recruits the Arp2/3 complex to existing actin filaments, promoting and stabilizing actin branching nuclei. Cortactin is important for promoting lamellae formation, invasive cell formation, cell migration, and endocytosis. Diseases associated with CTTN include sigmoid sinus adenocarcinoma and Wiskott-Aldrich syndrome. Pathways associated with CTTN include developmental Gap-Rob signaling and Syndecan-3-mediated signaling events. Gene ontology annotations associated with this gene include profilin binding. An important homologue of this gene is HCLS1. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.4 ★★★★★
Based on 8 reviews
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Product Reviews
★★★★★ 5
A great reference for Biblical factual archeology
Format: Paperback
I just received this book and I am so excited. It is a great tool and reference for Biblical studies. Each artifact has a great photograph next to the quick eye catching dates, discovery, period, keywords and Biblical passage. Then a brief but to the point description. It is simple and effective. Very easy to refer when reading your Bible or if you are just interested in archeology. Each artifact is about 2 pages and nothing more which is perfect for references. What a great book!
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Reviewed in the United States on March 1, 2025
★★★★★ 5
Excellent and in-depth archeologic finds that authenticate Bible history.
Format: Paperback
Archeology is proving much of the Bible's history as true. This book really delves into various sites and provides a lot of detail. My type of reading.
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Reviewed in the United States on February 21, 2026
★★★★★ 5
Great information and pictures
Format: Paperback
Great information on Biblical sites, beautiful pictures, and a pretty book as well. I gave several of these to my family for Christmas. Everyone seemed to love them. The only thing I will caution you about is that the book is small.
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Reviewed in the United States on January 22, 2026
★★★★★ 4
Knowledgeable and delectable book.
Format: Paperback
This book shows an excellent archaeological evidence of the Bible accuracy of places, names, events, etc. and proving for the Christians that the Bible is a historical document as well as the inspired inerrant word of God.
The majority of the book is interesting and delectable, I mean, the pages where the author presents archaeological facts such as the artifacts and their correlation with people, places times, events and practices recorded in the Bible. But I do not like when the author make personal assumptions and do not present any proof of that. He says: it probably be…. it may be… Here I show three cases of this conjectures;:
1.The author seems to affirm that the triumphal entry of Jesus into Jerusalem, or at least, the name of this event, was derived from the Roman triumph celebration. He says (page 231), regarding to the Roman Empire and the life of Jesus: “By the time of Jesus, the requirements and meaning of a triumphal entry had shifted slightly from its earlier roots associating it with a conquering hero, as it became even more significant and representative of kingship and divinity”. He continues to say: “In ancient Roman culture, a triumphant victor, known as vir triumphalis (“man of triumph”) would enter the city in a celebration parade wearing the laurel wreath and a purple garment, which identified him with the royal and the divine, while riding in a chariot pulled by four horses, alluding to Sol the sun god”.
But if we compare the Roman triumph celebration with the entry of Jesus into Jerusalem described in the Bible, it is not derived from the Roman culture but is the exact fulfillment of the prophesy of Zechariah 9:9. I do not see any correlation between the two celebration; Jesus did not ride a horse but a donkey to signify peace, meekness and humility. It was not a pompous entrance of a conquering hero or king wearing a expensive garment and royal crown.
2.Even though, it is not possible to identify the location of the tomb of Jesus with absolute certainty; the author states (page 199) that the tomb of Jesus is located at the Church of the Holy Sepulchre possible based on “the restoration work to the edicule and an arcosolium tomb from the Roman period found in the church of the Holy Sepulchre”. The author also affirms without giving any proof that: “Christians in Jerusalem then passed down a continuous memory of the location of the tomb (of Jesus) from the time of the burial and resurrection in AD33 until construction of the Church of the Holy Sepulchre was started in about AD326”. But where are the records of that time? The author probably based his statement on Eusebius who lived in the 3rd and 4th centuries AD.
According to the history , the Church of the Holy Sepulchre was built by the Roman Emperor Constantine around 326 AD, when her mother the Queen Helena, wanted to replace the pagan temples with Christian churches; she met the Bishop Macarious of Jerusalem who determined the location where Jesus had been buried at the place where was a temple to the Greek goddess Venus. At the beginning of the construction of the church, a rock-cut tomb was found there and an edicule was built to protect the site. But later the edicule was destroyed and rebuild. More tombs has been found under this church. How to be sure or verify that this rock-cut tomb was the one where Jesus was buried?
3.The author is biased with respect to the Masoretic Text, he make a statement but does not support it with any evidence in this regard. Writing about the Dead Sea Scrolls (Page 173) he states: “And certain passages in the Masoretic text seem to have been intentionally modified to match ideas and theology of medieval Judaism.” it is a bad accusation.
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Reviewed in the United States on April 23, 2021
★★★★★ 5
Great Worth
Format: Paperback
The Book That Must Be Read!
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Reviewed in the United States on April 18, 2026