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Description
Human rai14 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. 5. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. 6. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Retinoic Acid Induced 14 (rai14). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Retinoic Acid Induced 14 (rai14) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Retinoic Acid Induced 14 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Ankycorbin is a protein containing ankyrin repeats and coiled-coil domains, encoded by the RAI14 gene. It is expressed in multiple tissues and is thought to play a role in actin-regulated ectoderm specification, sperm polarity, and sperm adhesion. It may also promote the integrity of tight junctions in Sertoli cells of the blood-testis barrier. It has a predicted weight of 110.0 kDal and an isoelectric point of 5.87. It has a glutamine-rich region between nucleotides 914-978 and is otherwise rich in lycosides, glutamic acid, and serine, with less abundance of glycine and phenylalanine. It has four distinct isoforms (A, B, C, and D), most of which contain one or more splice variants, primarily differing in the 5' UTR. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.8 ★★★★★
Based on 9 reviews
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Product Reviews
★★★★★ 4
Title: Solid, Reliable Ink – No Frills, No Surprises (4 ★)
Style: Ink
The Bottom Line
The Epson 822 DURABrite Ultra Ink Standard‑Capacity Black & Color Cartridge Combo Pack (T822120‑BCS) does exactly what it promises: it prints clean, sharp pages on the WorkForce Pro WF‑3820/3823/4820/4830/4833/4834 series with no hiccups. It isn’t a game‑changing breakthrough, but it’s dependable enough that you can toss it in your cart, try it out, and feel confident you won’t be left with a printer jam or faded text.
Why I Gave It Four Stars
Aspect Verdict Comments
Performance ★★★★★ Prints consistently on both black and color pages. Colors are vivid, blacks are deep, and there are no streaks or banding.
Compatibility ★★★★★ Works flawlessly with all the WorkForce Pro models listed on the box. No driver updates or firmware tricks needed.Value ★★★★☆ Standard‑capacity cartridges are a bit pricier than high‑yield options, but you get the expected page yields (≈ 2,000 B / 1,800 C / M / Y).
Packaging & Arrival ★★★★★ Packaged securely, the “OME” seal was intact, and the cartridges arrived ready to install.
Innovation ★★☆☆☆ Nothing revolutionary – just solid, reliable ink. If you’re looking for a “wow” factor, this isn’t it.
Return Policy ★★★★★ Amazon’s 30‑day liberal return policy makes the purchase low‑risk; you can always send it back if it doesn’t meet your expectations.
Overall, the combo earns a four‑star rating because it meets every functional requirement without any drama, but it also doesn’t exceed expectations enough to merit a perfect score.
What I Actually Did
Unboxed the combo and confirmed the “OME” seal was intact – a quick visual check that the cartridges weren’t tampered with.
Installed the black cartridge first, then the four color cartridges, following the printer’s on‑screen prompts.
Ran a printer self‑test and printed a few typical office documents (reports, spreadsheets, a color flyer).
Checked the print quality side‑by‑side with my old OEM cartridges. The results were virtually identical – no ghosting, no color shifts.
Pros
Plug‑and‑play: No quirks, no firmware hacks.
Consistent quality: Straight‑forward, vibrant colors and clean blacks.
Reliable supply: Works across the entire WorkForce Pro lineup mentioned.
Risk‑free purchase: Amazon’s 30‑day return window lets you test without commitment.
Cons
Standard capacity only: If you print heavily, the higher‑yield (XL) versions would be more economical.
No “wow” factor: It’s a solid, work‑horse cartridge, not a revolutionary ink formula.
Price: Slightly higher than some off‑brand alternatives, though you pay for brand reliability.
Who Should Consider This
Home‑office users who need dependable daily printing without fuss.
Small businesses that run a mix of black‑and‑white documents and occasional color marketing pieces.
Anyone who values a hassle‑free experience and appreciates a generous return policy as a safety net.
If you’re hunting for an ink set that will wow you with cutting‑edge technology, look elsewhere. If you just want a cartridge that does its job, lasts as expected, and won’t surprise you with printer errors, the Epson 822 DURABrite Ultra combo is a safe bet.
Bottom line: It’s a reliable, as‑described product that earns my respect, just not my awe. Four stars—good enough to recommend, but not enough to call it a breakthrough.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 4, 2026
★★★★★ 5
Very good product and thanks for quality service.
Style: Ink
Arrived on time, good product and quality service. Thanks, and will buy again in the future.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 14, 2026
★★★★★ 5
Fast Delivery
Style: Ink
Fast delivery. Easy to install.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 12, 2026
★★★★★ 5
Our Standard Cartridges.
Style: Ink
These cartridges are the only ones that work for our Epson printer. in the past, I have tried 3rd party units with various degrees of success. Sometimes they will work and sometimes not. They are less expensive up front but, in the long run, I am going to stick with the genuine Epson product. They are the same price everywhere that I have shopped, so we purchase them from Amazon because of the free shipping. The Epson cartridges just work in our printer.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 4, 2026
★★★★★ 5
Bright, Dependable, and Jam-Free — Excellent Printer Paper!
Size: 1 Ream | 500 Sheets, Style: Letter (8.5x11)
The Amazon Basics Multipurpose Copy Printer Paper is a dependable and high-quality choice for all the daily printing needs, offering a bright white finish that makes text and images look sharp and professional. It feeds smoothly through the printer without any jams or misfeeds, which is essential for maintaining productivity throughout the day. The 20 lb weight feels sturdy and durable, making it perfect for everything from internal memos to important client documents. A great value-for-money purchase that provides consistent results every single time.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 23, 2026
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