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Description
Caspase 6 Activity Assay KitProduct Specification Usage Determination of pNA standard curve: 1. The 10 mM pNA standard was diluted with reaction buffer to 200, 100, 50, 25, 12. 5, 6. 25, 3. 125 M. Set the zero tube without pNA. 2. Take 100l of each concentration and add it to a 96 well plate or 100l cuvette, and measure the absorbance OD value at 405nm. 3. The OD405 value of each concentration standard tube is the x axis, and the corresponding pNA concentration is the y axis.
Product Specification
| Usage | Determination of pNA standard curve: 1. The 10 mM pNA standard was diluted with reaction buffer to 200, 100, 50, 25, 12.5, 6.25, 3.125 μM. Set the zero tube without pNA. 2. Take 100μl of each concentration and add it to a 96-well plate or 100μl cuvette, and measure the absorbance OD value at 405nm. 3. The OD405 value of each concentration standard tube is the x-axis, and the corresponding pNA concentration is the y-axis. Use Excel to make a standard curve of pNA concentration versus OD405 value. Detection of Caspase 6 enzyme activity: 1. Take out an appropriate amount of Ac-VEID-pNA (2mM) and place it on an ice bath for later use. 2. According to the 100ul reaction system: blank control group: 40μl of detection buffer, 0μl of sample to be tested, 50μl of lysate, 10μl of Ac-VEID-pNA (2mM); Sample group: 40μl of detection buffer, 50μl of sample to be tested, 0μl of lysate, 10μl3 of Ac-VEID-pNA (2mM). Add Ac-VEID-pNA (2mM) and mix well. Be careful to avoid bubbles during mixing. Incubate at 37 º C for 60-120 minutes. A405 can be measured when the color change is obvious. If the color change is not obvious, the incubation time can be appropriately extended, or even overnight. 4. The A405 of the sample minus the A405 of the blank control is the absorbance produced by pNA catalyzed by caspase 6 in the sample. By comparing the standard curves obtained in the above steps, it is possible to calculate the amount of pNA catalytically produced in the sample. |
| Theory | Based on Caspase 6, it can catalyze the substrate Val Glu Ile Asp p nitroanilide (Ac VEID pNA) to produce yellow pNA (p-nitroanilide), which can be used to detect the activity of Caspase 6 by measuring absorbance. pNA has a maximum absorbance at 405 nm. Caspase activity was obtained by measuring pNA using visible light colorimetry. Suitable for detecting Caspase 6 activity in mammalian tissues and cells. |
| Experiment Reagent Instrument | Microplate reader, spectrophotometer |
| Synonym | Mch-2,caspase-6,caspase 6 |
| Description | caspase 6 Activity Detection Kit is a kit that detects caspase 6 enzyme activity or purified caspase 6 enzyme activity in cell or tissue lysates by spectrophotometry. |
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4.5 ★★★★★
Based on 13 reviews
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Product Reviews
★★★★★ 5
A great toy for any dog!
Style: Small Ball, Size: 1 Count, Style: Small Ball, Size: 1 Count
The Hartz Duraplay ball is great! My 4 1/2 yr. old Pug has never liked playing with them before. He has gone crazy for this ball. He has no trouble grabbing it with is mouth. He ever gets it to squeak with his feet. I think it’s a great dog toy. Excellent!
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Reviewed in the United States on May 30, 2026
★★★★★ 5
Great Value and very durable
Style: Small Ball, Size: 1 Count
Very durable! We have purchased this for our new lab puppy. Highly recommend them. We have bought them before for our retrievers and it has been one of the longest lasting toys they own. Which says a lot because toys get torn up fast in our house. Definitely a good value and the material is easy on their teeth. Also it keeps ours busy for a while.
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Reviewed in the United States on June 11, 2026
★★★★★ 5
Great product! Durable, chewable, awesomeness!
Color: Grilled color, Color: Grilled color
Very well made and durable. My dog hasn't left them alone since i opened them. Hes been at it for about 45 minutes straight now! Lol. He's an avid chewer. He will chew thru and fabric or cloth toys in less then a day. But these are what he really likes because they take time to chew apart. The shapes, are great, because one he props up with his paws and chews and it seems like it's easy to grip for him. Thru are hard so if you have hard floors it can get a bit noisy when he drops them. Over all this is a great product. Safe, chewable, long lasting, fun shape looks real to him (i think lol), Me and my dog Lucky are both happy with his new toys!
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Reviewed in the United States on March 27, 2026
★★★★★ 5
Recommend for very heavy chewers
Color: Grilled color
My aggressive chewers love these! It’s been a few weeks and still in one piece and man they chew them for hours on end.
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Reviewed in the United States on May 26, 2026
★★★★★ 5
Amazingly great product!
Color: Grilled color
I’m not just saying this, ok, this is not hyperbole: this is THE best nylon bone for chewing that I have ever purchased. And you get TWO of them for relatively less expensive than most chew toys give you one! I have 2 golden retrievers, and if you know goldens, you know that they are some of the most relentless and powerful chewers around. So I can’t give them almost any toy other than rubber stuff or it will be destroyed in a period of time that doesn’t make it worth it. One of them even figured out how to destroy a supposedly indestructible toy made out of kevlar! She was able to get into it through the seam, which is not kevlar ha ha. Anyway, I digress; I never even considered nylon chews because they suck! Usually. Usually my dogs in the past have lost interest with them in less than a day. I took a chance on these mainly because of the novel shapes, the fact that they give you two, and the low price. Both my goldens LOVE these! Not just for one day, but going on weeks now! I don’t know if it’s the flavor or what, but I will see them gnawing on one of them at some point every day. And they CAN’T destroy them (I knew nylon was good for that, but it was the “who cares” attitude of my previous dogs toward nylon that kept me away). Both the “ribs” and “steak” (quite clever) are a bit worn on the edges now, but they look like they will be around for years. You can’t go wrong with this product (unless for some reason your dog does not like the flavor, but the flavor seems to be what draws my dogs to them). I highly recommend this (these) as the most cost-effective chew toy I’ve ever purchased.
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Reviewed in the United States on November 1, 2025
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