Human Osteoprotegerin/TNFRSF11B ELISA Kit

Detection Principle:

The double antibody sandwich ELISA method was used in this experiment. Anti human OPG monoclonal antibody is coated on the microplate. OPG in the sample and standard will bind to the antibody fixed on the plate, and the free components will be washed away; Biotin labeled anti human OPG multi antibody was added to combine with OPG bound on the microplate, and the free components were washed away; The streptavidin labeled horseradish peroxidase, which specifically recognizes biotin, is added to form a complex. After washing away the free components, the substrate solution (chromogenic agent) is added, and the color of the solution gradually turns blue. After adding the stop solution, the solution turns yellow and stops changing. The absorbance was measured with a microplate reader.

Detection Type: Double antibody sandwich method

Form: pre coated 96 well plate

Detection sample type: cell supernatant, serum, Plasma

Loading amount: 100ul

Kit components:

Pre coated 96 well plate, standard, anti human OPG detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 6.8 pg/ml

Detection Range: 62.5 - 4000 pg/ml

Recovery Range: 85.0-108.9%

Storage Method: 2-8 ℃ 

Background:

Osteoprotegerin (OPG), also known as OCIF and TNFRSF11B, is a secreted TNF superfamily receptor that regulates bone mineral density. OPG is widely expressed and released by mesenchymal stem cells, fibroblasts, and endothelial cells in the form of homodimers. OPG has the decoy receptor function of trace / Rank l and trail. OPG is involved in regulating the expression of trace on the cell surface and its ability to stimulate osteoclast formation. Dysregulation of OPG / trance interaction leads to Paget's disease, osteoporosis, bone loss, and vascular calcification.