Pay in installments of $56.25 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Jul 22 - Jul 27
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Mouse IL-1β ELISA KitProduct Specification Theory Double antibody sandwich enzyme linked immunosorbent assay was used in this kit. Specific anti mouse IL 1 antibody was precoated on a high affinity microplate. The standard substance, the test sample and the biotinylated detection antibody were added into the microplate Wells. After incubation, the IL 1 present in the sample combined with the solid phase antibody and the detection antibody to form immune complexes. After
Product Specification
| Theory | Double antibody sandwich enzyme-linked immunosorbent assay was used in this kit. Specific anti-mouse IL-1β antibody was precoated on a high-affinity microplate. The standard substance, the test sample and the biotinylated detection antibody were added into the microplate Wells. After incubation, the IL-1β present in the sample combined with the solid-phase antibody and the detection antibody to form immune complexes. After washing to remove the unbound material, the horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, the chromogenic substrate was added and the color was developed in the dark. The reaction was terminated by adding the termination solution, and the absorbance value was measured at 450nm wavelength (reference correction wavelength 540nm or 570nm). | |||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||
| Background | The interleukin-1 (IL-1) protein family contains the classical members IL-1α, Il-1β and IL-1ra, as well as IL-18, IL-33 and IL-1F5-10. IL-1α and IL-1β bind to the same cell surface receptor and share biological functions. "With the exception of skin keratinocytes, some epithelial cells, and certain cells of the central nervous system, unstimulated cells from healthy mice do not produce IL-1." "However, in response to inflammatory agents, infections, or microbial endotoxins, IL-1 is produced in large quantities by macrophages and a variety of other cells." IL-1β plays a key role in the physiological processes of immune response and inflammation, bone remodeling, fever, glucose metabolism, growth hormone /IGF-1. "Abnormal or sustained production of IL-1 has been associated with a range of pathological conditions, including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myeloid leukemia, insulin-dependent diabetes mellitus, atherosclerosis, nerve injury, and aging-related diseases." IL-1α and IL-1β have structurally related peptides that share approximately 25% identity at the amino acid level. They were synthesized as the size of 31 kDa precursor, and then cracking into mature protein, molecular weight is about 17.5 kDa. Caspase 1 / ICEI cracking IL - 1 beta precursor is a key step in the inflammatory response. Neither IL-1α nor IL-1β contain typical hydrophobic signal peptides, but there is evidence that these factors can be secreted through nonclassical pathways. Part of raw IL - 1 alpha can appear on the cell membrane, and may have biological activities. Unlike precursor IL - 1 alpha, beta IL - 1 precursor compared with mature body almost do not have biological activities. Both unprocessed and mature IL-1β can be exported from the cell. IL - 1 alpha and beta IL - 1 with IL - 1 ra combination immunoglobulin superfamily receptors play a role. "Cells expressing the 80-kda transmembrane type I receptor (IL-1RI) include T cells, fibroblasts, keratinocytes, endothelial cells, synovial fluid lining cells, chondrocytes, and hepatocytes." "Cells expressing the 68kDa transmembrane type II receptor (IL-1RII) include B cells, neutrophils, and bone marrow cells." "The two IL-1 receptors share approximately 28% identity in their extracellular domains, but a significant difference is found in their intracellular domains: the type II receptor has an intracellular domain of only 29 amino acids, whereas the type I receptor has an intracellular domain of 217 amino acids." IL-1RII does not produce a signal for IL-1 and may act as a decoy receptor to attenuate IL-1 function. IL-1 receptor accessory protein (IL-1RAcP) is associated with IL-1RI and required for IL-1RI signal transduction. IL-1ra is a secretory molecule that acts as a competitive inhibitor of IL-1. Soluble IL-1RI and IL-1RII can be detected in human plasma, joint synovial fluid, and conditioned media of a variety of human cell lines. In addition, IL-1binding proteins similar to soluble IL-1RII are capable of being encoded by cowpox and vaccinia virus. |
|||||||||||||||||||||||||||
| General Notes | 1. Please use the kit within the validity period. 2. Components of different kits and different batch kits should not be mixed. 3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with (1×) The samples were diluted and retested. If the cell culture supernatant sample needs to be diluted, except for the last step, diluent (1×) is used. "In addition to dilution, cell culture medium may be used for other intermediate dilutions." 4. The difference of the test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipettor, the washing technique, the reaction time or temperature, the storage of the kit, etc. 5. The termination solution in the kit is acidic. Please protect your glasses, hands, face and clothes when using it. 6. For scientific research only, not for in vitro diagnosis. |
|||||||||||||||||||||||||||
| Storage Temp. | Unopened kit, 2-8° C Storage | |||||||||||||||||||||||||||
| Test Range | 15.6pg/mL-1000pg/mL |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.5 ★★★★★
Based on 26 reviews
Sort
Product Reviews
★★★★★ 4
Base of you is very durable
Color: Blue
Overall this is a fun toy. My German Shepherd loves it. The ropes were chewed off within the first hour which was disappointing. Using it for treats did not work because the opening is so large. It has become only a chew toy and it is very durable. Since I bought this to enjoy the extra features, I am disappointed. I was able to buy three chew only toys for the same price. I still give it four stars because my dog likes it so much.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 15, 2023
★★★★★ 1
The dog will destroy the ropes and eat them be careful with this
Color: Blue
The rubber part is very durable but the strings or ropes are not. Puppy is eating the strings and having a hard time pooping them out. Just cut them off close to the knots and watch for them to eat the strings they pull off.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 12, 2022
★★★★★ 3
Is not as durable as they say for large dogs
Color: Multi-colored
My dog loved the fact he got a new toy and he loved the toy itself. Carrying it around and shaking it, that is.
The ropes are small and pop right back out of the ball. They are more for a small dog to tug on. The suction is strong but still not strong enough for a 100 lbs Rotti. He kept grabbing the eleasic ropes and pulling off the floor.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 20, 2023
★★★★★ 5
Even the rope is awesome!
Color: Multi-colored
The suction power on this wasn’t the greatest. Even using the sticky patch provided didn't keep it stuck to the floor. I will say though: I was terrified it would destroy the cheap flooring I put it on, but the sticky stuff didn’t harm the floor at all. The toy itself has been fantastic. My pup loves to chew on all parts of it (the ball, the suction piece, and the rope) and it’s held up against his jaws of steel. I will be purchasing another one of these once he’s finally destroyed the original (we’ve had it almost 3 months with just about daily use).
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on October 22, 2021
★★★★★ 5
Indestructible
Color: Blue
Best dog toy for my Daschund !
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on October 5, 2025