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Description
mIHC Dye (TSA770) Two-step MethodProduct Specification References 1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008. 9. 2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79 80, 105. Stack, E. C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46 58. 4.
Product Specification
| References | 1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. 2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79-80, 105. Stack, E.C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46-58. 4. Qian Bangguo Jiao Lei Application of multi-label immunofluorescence staining and Doppler imaging technology in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. |
| Theory | Multiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels. |
| Description | There are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases! Principle of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce an activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, replaced with a primary antibody for the second round of incubation, and replaced with another fluorescein substrate, so that multiple labeling can be achieved by reciprocating. |
| Composition | TSA monochromatic fluorescent dye 770 component A, TSA770 dye component B, signal amplification reaction solution |
| General Notes | 1. Formalin-fixed wax blocks or glass slides, large tissues or TMA, and sealing wax cannot be obviously damaged. 2. For glass slide samples, the tissue needs to be close to the glass slide to avoid wrinkles, and the glass slide must not be damaged, scratched or stained. 3. The tissue should contain at least more than 1000 cells. 4. The wax block needs to be embedded in solid tumor tissue, and necrotic tumor tissue, fine needle punctures, and cell slice samples will affect the staining effect. 5. The tissue should be fixed with 10% neutral formalin, and the normal fixation time is 18-24h. 6. The thickness of the slice is about 4um, and anti-detachment slides are used. It is recommended that the glass slides be prepared within one week after fixation. 7. Do not add any adhesive in the water bath slide fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove water, tap gently to remove water droplets, and do not wipe the slide with paper. 8. Place the glass slide on a hot plate at 45 °C for 30min (the natural air-drying time of the glass slide is not less than 1h). |
| Storage Temp. | Fluorescent dyes should be stored in the dark from light at 2-8 ℃, and the validity period is 12 months from receipt of the goods. |
| Applications | It is mainly used for scientific research on immunohistochemical staining of tissues, paraffin sections and TMA chips, and cannot be used for in vitro clinical diagnosis or human experiments. |
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4.7 ★★★★★
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★★★★★ 5
Ailun screen protector WORKS Great!
Model: iPad 11th A16 2025/10th, Model: iPad 11th A16 2025/10th
Lifesaver tempered glass cell phone protector. Smooth, thin and appears like the cell itself. Reactive to cell touch and no lag time.
I recently had a terrible accident from a heavy metal yeti mug fall onto my cell. I was livid when I realized my cell phone was sitting on the counter and was struck hard and thought my phone was severely damaged.
I remember I had placed the glass screen protector on and slowly peeled it off. To my happy amazed discovery, the Ailun screen protector truly protected my cell phone from damage. The stick on cover took the brunt of the metal mug falling 2-3 feet above my phone!!! Saved me alot of headache, money to replace the phone which was an important special gift, and anguish.
I will use this product again for future phones when I run out of cell life. Take a look of the cracked glass cover which helped me!!
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Reviewed in the United States on May 14, 2026
★★★★★ 5
Outstanding Value - Perfect for Everyday Use
Style: Wi-Fi, Size: 128GB, Color: Blue, Configuration: Without AppleCare+
This iPad delivers exceptional quality at an incredible price point! The battery life is absolutely remarkable - it easily handles full days of regular use without needing a charge. Whether I'm writing, browsing, or streaming videos, the performance stays consistently smooth and responsive.
What makes this iPad shine:
• Battery performance - Lasts all day through heavy writing sessions and video streaming
• Display quality - Crisp, vibrant Liquid Retina screen that's perfect for both work and entertainment
• A16 chip performance - Handles everything I throw at it without lag or slowdown
• Value proposition - Premium features at a surprisingly accessible price
For regular users who need reliable performance for writing, web browsing, and media consumption, this hits every mark perfectly. The build quality feels premium and the overall experience is seamless.
Bottom line: You honestly don't miss Apple Intelligence or other high-end features - this iPad does everything a typical user needs beautifully. If you're looking for solid performance, stable ipad with excellent battery life, and great value, this is your perfect match!
Highly recommend for anyone wanting iPad quality without the premium price tag!
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Reviewed in the United States on April 20, 2026
★★★★★ 5
It Does Exactly What You Expect an iPad to Do
Style: Wi-Fi, Size: 128GB, Color: Silver, Configuration: Without AppleCare+
It is an iPad, and it does all the iPad things exceptionally well.
The screen is bright and sharp, performance is fast and responsive, and the overall build quality is exactly what you would expect from Apple. Setup was simple, and everything worked right out of the box.
Whether you are browsing the web, watching videos, reading, gaming, or getting work done, it performs flawlessly.
Apple products are rarely the cheapest option, but they are consistently polished and reliable. If you are looking for a tablet that simply works and offers a premium user experience, this iPad is an excellent choice. Battery life is pretty good.
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Reviewed in the United States on May 14, 2026
★★★★★ 5
Worth the money
Style: Wi-Fi, Size: 128GB, Color: Pink, Configuration: Without AppleCare+
The battery lasts a long time, great quality. I use it for school and does everything I need it to. Good condition, charged quickly, and was easy to setup
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Reviewed in the United States on May 23, 2026
★★★★★ 5
Best tablet I’ve owned!
Style: Wi-Fi, Size: 128GB, Color: Blue, Configuration: Without AppleCare+
Fast, beautiful screen, and great battery life. The 11-inch size is the perfect middle ground for portability and productivity. Setup took less than 5 minutes.
I am incredibly impressed with this iPad. The A16 chip makes everything feel snappy, from multitasking to heavy gaming. The Liquid Retina display is vibrant and crisp, making it perfect for streaming videos. Setup was a breeze, and the battery life easily gets me through a full day of use. Highly recommend for students or professionals!
5 stars!
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Reviewed in the United States on May 15, 2026
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